| The establishment of an efficient and stable regeneration system for poplar leaves plays a crucial role in the success of genetic transformation in poplar trees. In this study, the leaves of tissue cultured seedlings of the elite poplar variety ‘Beilin Xiongzhu 1’ were used as explants. Tissue culture techniques were employed to investigate the effects of explant type, types and concentrations of plant growth regulators, TDZ treatment duration, leaf age, light quality, and other factors on the induction of adventitious buds. Microscopic observations were conducted on the regeneration process of adventitious buds, leading to the establishment and optimization of a leaf regeneration system. The results indicated that suitable leaf explants are those that are dark green, fully expanded, and 3~4 weeks old, specifically the 2nd to 4th young leaves below the shoot apex of rooting plantlets. The optimal induction medium consisted of 0.4 mg/L 6-BA + 0.05 mg/L NAA + 0.01 mg/L TDZ, with a TDZ treatment duration of 9~15 days. Under these conditions, the average adventitious bud induction rate reached approximately 85%, with a maximum of 94%. This study optimizes the tissue culture system for 'Beilin Xiongzhu 1' poplar and provides a reference for future research on its genetic transformation. |
