| To explore the effects of combined seed soaking and spraying with amino acid selenium fertilizer on the growth and quality of pea sprouts and determine the optimal application concentration, this experiment used pea seeds as the material. Different concentrations of amino acid selenium fertilizer were applied for seed-soaking treatment, and spraying treatment was conducted under the optimal seed-soaking condition. The optimal seed-soaking and spraying concentrations for pea sprouts were determined by measuring various growth and physiological indicators of pea sprouts. The results showed that low-concentration selenium fertilizer significantly promoted the growth of pea sprouts. The 75-fold dilution, with a selenium content of 30 mg/L, had the best seed-soaking effect, with sprout length and fresh weight reaching 14.55 cm and 0.36 g per sprout, respectively, which were 11.6% and 16.8% higher than those of the control, respectively. The selenium content reached 928.67 μg/kg, which was 3.5% higher than that of the control. Soluble protein and soluble sugar contents increased by 40.8% and 17.6%, respectively. Spraying with selenium fertilizer at different concentrations significantly increased the selenium content in sprouts, but spraying at higher concentrations inhibited sprout growth. Spraying with the 120-fold dilution, with a selenium content of 12 mg/L, increased the selenium content by 16.9% while maintaining good sprout growth and quality, with sprout length, soluble protein content, and soluble sugar content increasing by 13.6%, 2.7%, and 15.0%, respectively, compared with the control. Comprehensive analysis indicates that seed soaking with the 75-fold dilution followed by spraying with the 120-fold dilution of selenium-enriched fertilizer is the optimal selenium biofortification strategy for pea sprout production, as it improves selenium enrichment while maintaining favorable growth and quality. |