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| 自然失水胁迫对黄瓜幼苗的形态及生理特征的影响 |
| Effects of Natural Water Loss Stress on Morphology and Physiological Characteristics of Cucumber Seedlings |
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| DOI： |
| 中文关键词: 失水胁迫 黄瓜幼苗 棉子糖合成酶 水苏糖合成酶 |
| 英文关键词:Water loss stress Cucumber seedlings Raffinose synthase Stachyose synthase |
| 基金项目:河南省重大科技专项“设施主要果菜工厂化生产关键技术研究与示范”（241100110200）；河南省科技攻关项目“黄瓜优异种质资源的创制及轻简化新优品种选育”(252102111152);河南省国际联合实验室:河南省以色列蔬菜品种和配套技术。 |
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| 中文摘要: |
| 以黄瓜幼苗为试验对象，探讨了自然失水胁迫对其叶片中棉子糖合成酶（RS）和水苏糖合成酶酶活性及糖含量的影响，以及叶片可溶性蛋白、脯氨酸、丙二醛（MDA）含量和超氧化物歧化酶（SOD）与过氧化物酶（POD）活性等指标的变化。研究结果发现，在自然失水胁迫下，黄瓜幼苗含水量和生长形态指标均呈现下降趋势，至5~7 d时达到显著水平。棉子糖合成酶和水苏糖合成酶的酶活性均呈现持续下降趋势，其中棉子糖合成酶活性从0.14降至0.05，水苏糖合成酶活性从0.22降至0.08。在自然失水1 d后，水苏糖含量显著上升至对照组的2.4倍，之后有所下降。与此同时，棉子糖、蔗糖、葡萄糖和果糖的含量均显著增加。随着失水胁迫时间的延长，叶片中的可溶性蛋白、脯氨酸和MDA含量显著上升，而SOD和POD酶活性则表现出先上升后下降的趋势。这些结果表明，黄瓜幼苗在自然失水胁迫下，通过调节糖代谢和抗氧化系统来应对水分胁迫，以维持细胞的生理功能和结构完整性。 |
| 英文摘要: |
| In this study, we investigated the effects of natural water loss stress on the enzyme activities and sugar contents associated with raffinose synthase (RS) and stachyose synthase (STS), as well as the changes in leaf soluble protein, proline, malondialdehyde (MDA) content, and the activities of superoxide dismutase (SOD) and peroxidase (POD), using cucumber seedlings as the test subjects. It was found that under natural water loss stress, cucumber seedlings showed a decreasing trend in water content and growth morphology indices, which reached significance by 5~7 days. The gene expression of raffinose synthase (RS) increased at the beginning of stress and then decreased, whereas the gene expression of stachyose synthase (STS) did not change much initially but decreased at later stages. Correspondingly, the enzyme activities of RS and STS showed a continuous decreasing trend, with RS activity decreasing from 0.14 to 0.05 and STS activity decreasing from 0.22 to 0.08. The stachyose content increased significantly to 2.4 times that of the control after 1 day of natural water loss and then decreased. Meanwhile, the contents of raffinose, sucrose, glucose, and fructose also increased significantly. With prolonged water loss stress, soluble protein, proline, and MDA contents in the leaves increased significantly, while SOD and POD activities tended to increase initially and then decrease. These results suggest that cucumber seedlings respond to water stress by regulating sugar metabolism and antioxidant systems to maintain cellular physiological function and structural integrity under natural water loss stress. |
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