|
| 莴苣Shaker型钾通道的全基因组分析 |
| Genome-wide Analysis of Shaker Potassium Channels in Lactuca sativa L |
| |
| DOI： |
| 中文关键词: 莴苣 Shaker基因家族 生物信息学 结构预测 顺式作用元件分析 |
| 英文关键词:Lactuca sativa L. Shaker gene family Bioinformatics Structure prediction Cis acting element analysis |
| 基金项目:陕西省重点研发计划（2024NC-YBXM-058）；陕西省教育厅重点项目（24JR002）；安康市中青年科技创新领军人才计划（AK2024PD-ZQN-02）；安康市科技计划（AK2023-NY-13）；陕西省高校青年创新团队（2023AYTD01）；大学生创新训练（202211397007；S202311397020；202411397034）。 |
|
| 摘要点击次数: 68 |
| 全文下载次数: 43 |
| 中文摘要: |
| 采用生物信息学方法对莴苣Shaker基因家族成员染色体定位、基因结构、蛋白质理化性质、保守基序及结构域、系统进化关系、蛋白质二、三级结构、启动子顺式作用元件进行研究。结果表明本研究鉴定到12个莴苣Shaker基因家族成员。它们分布在6条染色体上，其编码氨基酸长度为631~915aa，分子量介于72.64~102.97 kD之间，等电点为6.05~8.32，均为亲水性蛋白。亚细胞定位预测显示该基因家族定位在细胞质膜上,其中LsShaker4、LsShaker7、LsShaker11定位在叶绿体上。莴苣结构分析结果显示，各类群基因具有相似的内含子/外显子结构，且外显子数目均稳定在9~13之间。因此,根据蛋白二、三级结构预测分析发现莴苣中包含内向整流钾离子通道LsShaker6、LsShaker7、LsShaker10、LsShaker11，弱整流钾离子通道LsShaker8，外向整流钾离子通道LsShaker1、LsShaker2、LsShaker3、LsShaker5、LsShaker12。根据顺式作用元件分析结果识别出了光响应、激素响应、胁迫响应等相关的顺式作用元件，推测该基因家族可能参与植物环境变化的适应、激素调控下的生长发育、以及胚乳和种子发育等生理过程。 |
| 英文摘要: |
| To investigate the gene family structure and functional characteristics of Shaker type potassium channels in Lactuca sativa L. and to provide a basis for investigating their roles in lettuce growth and development, bioinformatics methods were employed to analyze chromosomal distribution, gene structure, protein physicochemical properties, conserved motifs and domains, phylogenetic relationships, predicted protein secondary and tertiary structures, and promoter cis acting elements of the Lactuca sativa Shaker gene family. A total of 12 Shaker genes were identified, which were unevenly distributed across six chromosomes. The encoded proteins ranged from 631 to 915 amino acids in length, with molecular weights of 72.64–102.97 kDa and predicted isoelectric points ranging from 6.05 to 8.32, and all were identified as hydrophilic proteins. Subcellular localization prediction indicated that members of this gene family were primarily localized to the plasma membrane, while LsShaker4, LsShaker7, and LsShaker11 were predicted to localize to the chloroplast. Structural analysis revealed that genes from different groups shared similar intron–exon structures, with exon numbers consistently ranging from 9 to 13. Based on predicted protein secondary and tertiary structures, Lactuca sativa was found to contain inward rectifying potassium channels (LsShaker6, LsShaker7, LsShaker10, and LsShaker11), a weakly rectifying potassium channel (LsShaker8), and outward rectifying potassium channels (LsShaker1, LsShaker2, LsShaker3, LsShaker5, and LsShaker12). Analysis of cis acting elements identified regulatory elements associated with light responsiveness, hormone responsiveness, and stress responsiveness, suggesting that this gene family may participate in plant adaptation to environmental changes, hormone regulated growth and development, as well as endosperm and seed development. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
|
|
