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| 陕西安康魔芋病毒小RNA测序及病毒全基因组扩增 |
| Small RNA Sequencing and Whole Genome Amplification of Dasheen Mosaic Virus Infecting Konjac in Ankang, Shaanxi |
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| DOI： |
| 中文关键词: 魔芋病毒 小RNA 全基因组 基因重组 |
| 英文关键词:Konjac virus Small RNA Whole genome Gene recombination |
| 基金项目:陕西省重点研发计划（2024NC-YBXM-058）；陕西省教育厅重点项目（24JR002）；安康市中青年科技创新领军人才计划（AK2024PD-ZQN-02）；安康市科技计划（AK2023-NY-13）；陕西省高校青年创新团队（2023AYTD01）；大学生创新训练（202211397007；S202311397020；202411397034）。 |
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| 中文摘要: |
| 通过小RNA（small RNA, sRNA）测序技术对陕西安康魔芋病毒病进行鉴定，序列比对显示鉴定到了芋花叶病毒（dasheen mosaic virus, DsMV）。采用长链RT-PCR和RACE技术获得了DsMV安康魔芋分离物全基因组序列，序列分析结果显示DsMV全基因组长度为9 972 nt，SDT序列一致性表明,DsMV安康魔芋分离物与已发布的DsMV全基因组序列一致性仅有78.4%～85.4%，存在较大的分子变异；进化树显示,DsMV安康魔芋分离物与来自埃塞俄比亚的芋头分离物聚集在一起，亲缘关系最近。此外，RDP5检测到5个显著的重组事件，表明DsMV在天南星科植物中存在广泛的基因重组。该研究结果为魔芋病毒病的分子检测、病害测报和综合防治提供了科学依据。 |
| 英文摘要: |
| Small RNA sequencing was used to diagnose viral disease in konjac in Ankang, Shaanxi, and the results revealed infection by dasheen mosaic virus (DsMV). The complete genome sequence of a DsMV isolate from Ankang was obtained using long-range RT-PCR and RACE. Sequence analysis showed that the DsMV genome was 9,972 nt in length. SDT analysis indicated that DsMV isolates from konjac in Ankang shared only 78.4%-85.4% nucleotide sequence identity with published DsMV genome sequences, indicating substantial molecular divergence. Phylogenetic analysis showed that the DsMV isolates from Ankang clustered with a taro isolate from Ethiopia and were most closely related to it. In addition, RDP5 analysis detected five significant recombination events, suggesting extensive recombination in DsMV populations infecting Araceae hosts. These findings provide a scientific basis for molecular diagnosis, disease forecasting, and integrated management of DsMV-associated disease in konjac. |
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