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| 榆林市5个优势品种桑树的1-脱氧野尻霉素含量变化规律研究 |
| Variation Patterns of DNJ Content in Five Superior Mulberry Varieties from Yulin City |
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| DOI： |
| 中文关键词: 桑树 品种 DNJ 采收时间 采收部位 |
| 英文关键词:Mulberry tree Variety DNJ Harvesting time Collection site |
| 基金项目:陕西林业科技创新重点专项（SXLK2023-02-36，SXLK2023-02-13）；榆林市科技局项目（2023-CXY-171）;安康学院陕西省蚕桑重点实验室项目(2025AYCSKFKT02);榆林学院高层次青年科学启动基金（18GK08）；榆林市首届“科学家+工程师”队伍项目(YLKG-2022-3);陕西省科技创新团队项目（2025KS-CXTD-038）。 |
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| 中文摘要: |
| 采用高效液相色潽－紫外检测法对不同采收时间的桑树品种（吴堡桑、丰驰2号、丰驰1号、选七九二、荆桑）的各器官（叶、桑枝皮层、桑木质部）不同部位中1-脱氧野尻霉素进行定量检测，探究DNJ分布和变化规律，以期筛选出1-脱氧野尻霉素含量最高的桑树品种，明确其DNJ富集部位和最佳采收时间。结果发现：吴堡桑是最优的采收树种，7月底是吴堡桑的最佳采收期。比较吴堡桑不同器官、不同部位DNJ含量，发现不同器官、不同部位之间含量差异显著，DNJ富集主要在桑叶的顶芽和桑枝皮层的下段皮。研究结果可为榆林地区桑树的1-野尻霉素含量变化规律研究和生产采收提供理论依据和技术指导。 |
| 英文摘要: |
| The 1-deoxynojirimycin (DNJ) content in different tissues (leaves, branch bark cortex, and xylem) and sub-tissues of five mulberry varieties (‘Wubao’, ‘Fengchi No.2’, ‘Fengchi No.1’, ‘Xuan 792’, and ‘Jingsang’) was quantified using high-performance liquid chromatography with ultraviolet detection (HPLC-UV) to investigate DNJ distribution patterns and temporal dynamics. This study aimed to identify the variety with the highest DNJ content, determine DNJ-enriched tissues, and define optimal harvesting times. Key findings are as follows:Optimal variety and harvesting period: ‘Wubao’ exhibited the highest DNJ accumulation, with late July identified as the optimal harvesting period. Tissue and sub-tissue specificity: Significant differences in DNJ content were observed among tissues and sub-tissues. DNJ was predominantly enriched in the apical buds of leaves and the lower cortex of branch bark in ‘Wubao’. Temporal variation: DNJ accumulation peaked in late July, aligning with the physiological maturation stage of mulberry tissues. These results provide a theoretical foundation and practical guidelines for DNJ-oriented mulberry cultivation and harvesting strategies in the Yulin region. The findings highlight the potential of ‘Wubao’ as a priority cultivar for industrial DNJ extraction, particularly targeting its apical buds and lower bark cortex during late July. |
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