| Walnut rot is a major disease affecting walnut trees in the western Henan region. Identifying the pathogens of the disease and investigating the control technology are helpful for controlling the occurrence and harm of the disease. In this study, methods including pathogen isolation, culturing, purification, and indoor identification, as well as field drug trials, identified the causative pathogen and evaluated the effectiveness of various control measures. The pathogen responsible for walnut rot in this region was identified as Cytospora juglandicola. The colonies of the pathogen on agar plates were round or oval in shape, and its conidia were single-celled, colorless, and banana-shaped, measuring （1.9~2.9）μm×（0.4~0.6）μm. Laboratory tests indicated that scraping the affected areas and applying certain treatments effectively controlled the disease. The most effective treatments included 3% humic acid (≥40 g/L) and 0.5% amino oligosaccharin at 33.33 μg/L, both achieving 100% efficacy. Mixing dry fertilizer with water in a 22∶1 ratio and applying it to the affected areas helped promote wound healing and tree vigor recovery. It is recommended to apply these treatments during the spring peak of disease incidence to ensure maximum effectiveness. |
