| Multivariate statistical method was used to analyze the content characteristics of nine alkaloids in Pinellia ternata cultivated in different regions of Gansu province,and the relationship between alkaloid content and geographical origin was explored. High-performance liquid chromatography (HPLC) was used to determin the concentrations of nine alkaloids,including trigonelline,nicotine,5’inosinic acid,uridine,adenosine,inosine,vernine,thymidine and ephedrine in 39 pinellia samples from four main producing areas in Gansu Province. Non-parametric test,correlation analysis,principal component analysis,cluster analysis and discriminant analysis were used to analyze and evaluate the relationship between alkaloid content and geographical origin. The results showed that,except for the content of 5’Inosinic acid,which followed a normal distribution,the other eight alkaloids were skewed. The contents of nicotine,trigonelline,uridine,and 5’inosinic acid were relatively higher than those of the other alkaloids. .The Kruskal Wallis test showed significant differences in alkaloid content among Pinellia ternata samples from different producing areas (P<0.05). The alkaloid content was higher in Qinzhou District and Xihe County. Correlation analysis revealed complex interrelationships among alkaloid components. Using an eigenvalue greater than 1 as the criterion,three principal components were extracted through principal component analysis,with a cumulative variance contribution rate of 75.2%. The results of cluster analysis were largely consistent with those of the Kruskal-Wallis test and principal component analysis. The results of OPLS-DA analysis and principal component analysis mutually confirmed that nicotine,trigonelline,uridine,and 5′-inosinic acid were the main characteristic alkaloid components of Pinellia ternata. In conclusion,the alkaloid quality characteristics of pinellia pinellia from different producing areas in Gansu province exhibit significant differences. This study provides scientific basis for identification,quality control and high quality development of pinellia from Gansu Province. |
