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| 玉米自交系穗部性状的全基因组关联分析 |
| Genome-Wide Association Study of Ear Related Traits in Maize Inbred Lines Water-Saving for Crops in Arid Area of Northern Shaanxi, Yulin , Shaanxi 719000, China) |
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| DOI： |
| 中文关键词: 玉米自交系 穗部性状 SNP 群体结构 全基因组关联分析 |
| 英文关键词:Maize inbred lines Ear traits SNP Population structure GWAS |
| 基金项目:国家自然科学基金项目（32060489）；榆林市科技局项目（2019-106-5）。 |
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| 摘要点击次数: 94 |
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| 中文摘要: |
| 玉米穗部性状显著影响产量，通过全基因组关联分析(GWAS)挖掘与穗部性状紧密关联的遗传位点，不仅有助于理解这些性状的遗传基础，还可以开发分子标记，为分子标记辅助选择育种奠定基础。本研究选用350份玉米自交系构成的自然群体，利用玉米6H60K芯片鉴定自交系的基因型。采用混合线性模型（MLMM）对穗行数、穗轴粗、穗长、行粒数和穗粗等性状开展全基因组关联分析，筛选与目标性状关联的SNP标记。研究结果显示，五个穗部性状在试验群体中均呈现正态分布特征，两个环境下各性状的变异系数均大于10%，5个性状的偏度和峰度值均在-1~1之间；以P<0.000 1为标准，共获得32个与玉米穗部性状显著相关的SNP位点，分布于1~10号染色体上。结果表明，玉米自交系穗部性状作为典型的数量性状，主要受微效多基因控制，遗传基础复杂。 |
| 英文摘要: |
| Maize ear traits play an important role in determining yield. Genome-wide association studies (GWAS) can help find genetic sites related to these traits, which improves understanding of their genetic basis and supports the development of molecular markers for breeding. In this study, a total of 350 maize inbred lines were used, and their genotypes were identified using the maize 6H60K chip. A mixed linear model (MLMM) was applied to study key ear traits, including ear row number, cob diameter, ear length, kernel number per row, and ear diameter, and to identify SNP markers linked to these traits.The results showed that all five ear traits followed a normal distribution in the tested population. The variation coefficients for each trait were greater than 10% in both environments, and the skewness and kurtosis values ranged between -1 and 1. Using a significance level of P < 0.0001, a total of 32 SNP loci related to maize ear traits were found across chromosomes 1 to 10. These findings suggest that maize ear traits are quantitative traits mainly controlled by many small-effect genes and have a complex genetic background. |
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