Using ‘Benihoppe’ strawberry as the test material, this study explored the effects of different types of stolon seedlings, substrate compositions, and nutrient solution formulations on the survival and growth of stolon seedlings in plug trays. The aim was to provide technical recommendations for plug tray propagation of strawberry stolon seedlings. The results showed that stolon seedlings with few initial roots were most suitable for plug tray propagation, achieving high survival rates and robust growth, followed by seedlings with a convex base. When comparing different substrate compositions, pure peat resulted in the lowest survival rate (52.7%), significantly lower than the other five substrate treatments. Between 34 and 55 days after cutting, leaf area in the peat-to-vermiculite 1∶1 mixture was significantly larger than in the pure peat treatment. At 55 days, no significant differences in leaf area were observed among other substrate mixtures, all of which significantly outperformed pure peat. Substrates containing peat, vermiculite, and perlite, excluding pure peat, were suitable for plug tray propagation of ‘Benihoppe’ stolon seedlings. Two weeks after cutting, spraying with the garden trial universal formula, Yamazaki strawberry formula, or Hoagland universal formula nutrient solution significantly promoted the growth of stolon seedlings. Between 20 and 55 days after cutting, plant height, leaf number, and leaf area in these treatments were significantly higher than those in the control, with no significant differences among the three nutrient formulations. In conclusion, stolon seedlings with initial roots or a convex base, propagated in a substrate mixture of peat, vermiculite, and perlite, and sprayed with specific nutrient solutions after establishment, can effectively enhance the survival and growth of strawberry seedlings. |